Date :
Wei-Hao Liao submitted on 21-May-2021
Application Tested :
IHC (Free Floating)
Sample Species :
Ms
Sample :
Mouse Brain 250μm slice
Fixation :
4% PFA in PBS, 4°C, 16Hr
Permeabilization /Antigen retrieval :
2% Triton X-100 in PBS, 25°C, 48Hr
Primary Antibody :
1:100, 4°C, 48Hr
Figure Note :
Mouse Brain "250μm slice"
Ziess LSM780 confocal microscopy.
1st & 2nd Abs diluted in SignalStain® Antibody Diluent.
Blocking agent: 10% normal goat serum, 1% triton-X100, 2.5%DMSO, 0.2% sodium azide in PBS
Washing buffer: 3% NaCl, 0.2% TritonX-100 in PBS
Wash 4ºC 2 days.
After stain, samples( 250um slices) incubated in RapiClear®1.47 60min for tissue clearing, and then mounted (also in RapiClear®) for imaging.